Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Anti-inflammatory effects of kaempferol, myricetin, fisetin and ibuprofen in neonatal rats

Peng Guo, Yun-Yun Feng

The Second Pediatric Department of Internal Medicine, Zhumadian Central Hospital, Zhumadian, No. 747 Zhonghua Road, Zhumadian, Henan Province 463000, China;

For correspondence:- Yun-Yun Feng Email: yunyunfeng1@163.com Tel:+863962726840

Received: 9 September 2016 Accepted: 14 July 2017 Published: 31 August 2017

Citation: Guo P, Feng Y. Anti-inflammatory effects of kaempferol, myricetin, fisetin and ibuprofen in neonatal rats. Trop J Pharm Res 2017; 16(8):1819-1826 doi: 10.4314/tjpr.v16i8.10

© 2017 The authors.
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Abstract

Purpose: To investigate the anti-inflammatory effects of kaempferol, myricetin, fisetin and ibuprofen in rat pups.

Methods: The expression levels of cyclooxygenase (COX)-1, COX-2 and tumour necrosis factor-α (TNF-α) were determined by western blotting; the inhibition of these proteins by plant compounds was evaluated. In addition, a computational simulation of the molecular interactions of the compounds at the active sites of the proteins was performed using a molecular docking approach. Absorption, distribution, metabolism and excretion (ADME) and toxicity analysis of the plant compounds was also performed.

Results: Kaempferol, myricetin and fisetin inhibited the activities of COX-1, COX-2 and TNF-α by 70–88 %. The computational simulation revealed the molecular interactions of these compounds at the active sites of COX-1, COX-2 and TNF-α. ADME and toxicity analysis demonstrated that the three plant compounds were safe.

Conclusion: The data obtained indicate that myricetin, kaempferol and fisetin exert anti-inflammatory effects in neonatal rats, with fewer side effects than those of ibuprofen.

Keywords: Non-steroidal anti-inflammatory drugs, Cyclooxygenase-1, Cyclooxygenase-2, Tumour necrosis factor-^5;